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utilizes just one metallic sure only in to the β-Website. certainly by far the most major case in point in the amidohydrolase superfamily is uronate isomerase from E. coli
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there isn't any proof to propose that Glu-131 performs any operate in catalysis in addition to to assist coordinate the sole divalent cation. The enzyme is cost minimal through the cleavage in the amide bond.
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Mutation of Y223 into a phenylalanine residue resulted inside of a mutant with just about precisely the same catalytic motion because the wild sort enzyme and so this residue just isn't substantial for that binding of substrate in NagA.
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